Separates analytes from a liquid sample by selectively retaining them on a solid sorbent while allowing the rest of the sample to pass through.
Mechanism:
Analyte retention is based on interactions (like adsorption, ion exchange, or partitioning) with the sorbent's surface.
Key Features:
Versatile sorbents:Available in a wide range of materials for different applications, including silica, polymeric, Florisil, and specialty options like CarboPrep and EPH sorbents.
High purity:Manufactured with clean sorbents and materials to minimize interference.
Reproducibility:Rigorous quality control ensures consistent performance.
Formats:Available in cartridges and 96-well plates.
Supported Liquid Extraction (SLE)
Function:
An efficient form of liquid-liquid extraction in a miniaturized format that removes proteins, phospholipids, and salts from samples.
Mechanism:
The sample is loaded onto a highly porous diatomaceous earth support, where it diffuses. An immiscible solvent is then added, and the analytes partition between the aqueous and organic phases, followed by elution.
Key Features:
Simplicity:Faster and easier than traditional liquid-liquid extraction; simply load, wait, and elute.
Effective:Quickly removes interferences like proteins and salts.
High-throughput:Easily automated for high-throughput laboratories.
Versatility:Suitable for a wide range of sample matrices and analyte types.
Formats:Available in cartridges and 96-well plates.