Aminoglycoside Analyzer
An aminoglycoside analyzer is an analytical instrument used to measure the concentration of aminoglycoside antibiotics in a variety of samples. These systems are used for quality control in pharmaceutical manufacturing and for therapeutic drug monitoring in clinical settings.
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Product Description
An aminoglycoside analyzer is an analytical instrument used to measure the concentration of aminoglycoside antibiotics in a variety of samples. These systems are used for quality control in pharmaceutical manufacturing and for therapeutic drug monitoring in clinical settings.
Technical specifications
Analyzers use different methods depending on the application. Common technologies include:
- High-Performance Liquid Chromatography (HPLC) with specialized detection: Many analyzers use HPLC, a technique that separates components in a mixture, to isolate specific aminoglycosides. Because these antibiotics do not have strong chromophores, they require specialized detection methods.
- Pulsed Amperometric Detection (PAD): PAD is a sensitive electrochemical detection method. In one example, chromatography is followed by the post-column addition of a sodium hydroxide solution to prepare the aminoglycosides for detection in an electrochemical cell.
- Mass Spectrometry (MS): Mass spectrometers detect and quantify the separated compounds by measuring their mass-to-charge ratio. In some setups, no chromatography is required, using electrospray ionization-mass spectrometry (ESI-MS) for rapid determination.
- Immunoassays: These tests use antibodies that specifically bind to aminoglycosides to determine their concentration. Techniques include enzyme-linked immunosorbent assay (ELISA) and chemiluminescence immunoassay (CLIA). They are valued for their high throughput and sensitivity.
- Capillary Electrophoresis (CE): This method uses an electric field to separate components based on their charge and size. It can be used as an alternative to HPLC.