Nexera lite inert
The analysis of proteins such as antibody drugs requires careful operation because a high-salt mobile phase sometimes corrodes the system. In addition, the adsorption of proteins to metal surfaces often results in poor chromatographic performance.
Inert High Performance Liquid Chromatograph (HPLC) System
Providing the same expandability and superior performance advantages of the Nexera series HPLC Systems, the Nexera lite inert is the ideal solution for addressing challenges related to metal-adsorption that compromise quantitative accuracy, sensitivity, or separation and mobile phases that cause system durability problems. It is a robust HPLC system that eliminates the risk of corrosion by high-salt mobile phases and sample adsorption onto metal surfaces with a maximum pressure tolerance of 20 MPa. It also improves the data quality of protein analysis in biological samples with superior reproducibility, without any special considerations for stable and long-term use.
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Product Description
The analysis of proteins such as antibody drugs requires careful operation because a high-salt mobile phase sometimes corrodes the system. In addition, the adsorption of proteins to metal surfaces often results in poor chromatographic performance.
Inert High Performance Liquid Chromatograph (HPLC) System
Providing the same expandability and superior performance advantages of the Nexera series HPLC Systems, the Nexera lite inert is the ideal solution for addressing challenges related to metal-adsorption that compromise quantitative accuracy, sensitivity, or separation and mobile phases that cause system durability problems. It is a robust HPLC system that eliminates the risk of corrosion by high-salt mobile phases and sample adsorption onto metal surfaces with a maximum pressure tolerance of 20 MPa. It also improves the data quality of protein analysis in biological samples with superior reproducibility, without any special considerations for stable and long-term use.
Technical specifications
Improved Data Reliability by Preventing System Corrosion from Halogenic Salt
For analysis of antibody drugs or proteins in biological samples,a metal-free LC system is required for good repeatability over a long time due to high-concentration, salt-containing mobile phases such as Sodium chloride. With no metal material in the low path, Nexera lite inert provides stable aggregate assay results with good repeatability.
Superior Retention Time Stability by Highly Accurate Solvent Delivery
With the outstanding flow-rate stability, the Nexera lite inert provides excellent retention time repeatability.
Real-Time Monitoring of Mobile Phase pH
In ion exchange or size exclusion chromatography, the pH of the mobile phase sometimes affects the separation of compounds. The pH Monitor, pHM-40, continuously monitors and records the pH of the mobile phase. The pH and detector chromatograms are saved within the same data file and can be overlapped, ensuring efficient data traceability.
Analysis of Antibody Drugs by Gel Filtration Chromatography
Antibody aggregates indicate the immunogenic potential. In addition, aggregates can result in lower purification efficiency and lower production quantities of antibodies, which are the main active ingredient in antibody drugs. Therefore, antibody drug aggregate analysis is an important process in the manufacturing and quality control of biopharmaceuticals. Using a Nexera lite inert system ensures reliable data can be acquired without worrying about protein adsorption or the risk of corrosion by high salt concentrations.
Analysis of Antibody Drugs by Gel Filtration Chromatography
Antibody aggregates indicate the immunogenic potential. In addition, aggregates can result in lower purification efficiency and lower production quantities of antibodies, which are the main active ingredient in antibody drugs. Therefore, antibody drug aggregate analysis is an important process in the manufacturing and quality control of biopharmaceuticals. Using a Nexera lite inert system ensures reliable data can be acquired without worrying about protein adsorption or the risk of corrosion by high salt concentrations.
Purifying and Selectively Recovering Fluorescently Labeled Antibodies
Using a combination of multiple detectors and fraction collectors offers the feexibility to purify samples based on the specific characteristics of target compounds. For example, by using both an absorbance detector and fluorescence detector, antibodies with and without fluorescent labeling can be monitored at the same time. Furthermore, by using an FRC-10A fraction collector to ensure reliable preparative separation of only target components, fluorescently labeled antibodies can be used directly in the next process step.